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Application Notes
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Immunohistochemistry Dilution: 1:200 - 1:1,000 Application Note: This product has been assayed against 1.0 μg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 μg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000
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