产品详情
所在位置: 首页> 产品目录> 二抗>
查看大图

  • 产品名称：Goatanti-HumanImmunoglobulinkappaChainComplex(Igk)Antibody

  • 产品型号：
  • 产品厂商：BioLeaf
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
Goatanti-HumanImmunoglobulinkappaChainComplex(Igk)Antibody
详情介绍：
Immunogen

Immunogen: Human kappa light chain
Isotype IgG
Characteristics The anti-Human κ (kappa chain) Antibody detects the kappa chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain.
Purification Human κ (kappa chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. Specificity for kappa chain was confirmed by ELISA minimal cross reactivity against other human heavy or lambda light chain isotypes.
Alternative Name kappa )
Application Notes

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Human κ (kappa chain) Antibody is suitable for immunoassays where specificity to the immunoglobulin kappa subuint is desired. Antibody has been tested by ELISA, western blot immunoblot, and immunohistochemisty. Optimal concentrations in immunoassays should be determined by the researcher.

Western Blot Dilution: 1:1,000 - 1:10,000

ELISA Dilution: 1:10,000 - 1:20,000
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiry Date 12 months