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  • 产品名称：CerebellarPedunclesLysate

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  • 产品厂商：AvivaSysBio
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CerebellarPedunclesLysate
详情介绍：
Characteristics Human cerebellar peduncles tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 °C. The human cerebellar peduncles tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the cerebellar peduncles tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The cerebellar peduncles tissue is then Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.
Application Notes Cerebellar Peduncles lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel
Restrictions For Research Use only
Handling Advice Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Storage 4 °C/-80 °C
Storage Comment Store at 2-8 °C for continuous use. For extended storage, freeze working aliquots at -70 °C.
Expiry Date 6 months