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  • 产品名称：RectumMembraneLysate

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  • 产品厂商：AvivaSysBio
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RectumMembraneLysate
详情介绍：
Characteristics Human rectum tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human rectum tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 °C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated rectum tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated rectum tissue membrane protein is then Western analyzed by either GAPDH or beta-actin antibody to confirm there is no signal or very weak signal.
Application Notes Rectum membrane protein lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel
Restrictions For Research Use only
Handling Advice Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Storage 4 °C/-80 °C
Storage Comment Store at 2-8 °C for continuous use. For extended storage, freeze working aliquots at -70 °C.
Expiry Date 6 months