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  • 产品名称：JurkatWholeCellLysate

  • 产品型号：
  • 产品厂商：AvivaSysBio
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JurkatWholeCellLysate
详情介绍：
Specificity Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents.
Application Notes The cells were grown in Dulbecco's medium supplemented with 10 % fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0 % NP-40, 0.5 % sodium
Restrictions For Research Use only
Handling Advice Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Storage -20 °C/-80 °C
Storage Comment Store vial at -70 °C or COLDER. ﾠ For extended storage, aliquot contents to minimize freeze/thaw cycles. ﾠ Expiration date is three (3) months from date of opening if stored at -20 °C or (1) year from date of opening if stored at -70 °C.
Expiry Date 6 months