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  • 产品名称：MouseIgGIsotypeControl

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  • 产品厂商：BioLeaf
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简单介绍：
MouseIgGIsotypeControl
详情介绍：
Isotype IgG
Fragment Whole molecule
Characteristics Mouse IgG2b control (Whole Molecule), is suitable to be used as control, standard, blocking agent, or coating protein in a variety of assays, including ELISA, immunobloting (Dot blot and Western blot), immunoprecipitation, immunodiffusion, and immunoelectrophoresis. It may also be used as antigen or ligand in immunochemical conjugation reaction. The Mouse IgG2b control (Whole Molecule)is highly purified from normal mouse ascites through Protein A affinity column and produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes.
Purification Protein A affinity column
Research Area Immunology, Secondary Antibodies
Application Notes Each Investigator should determine their own optimal working dilution for specific applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to make a concentration of 1 mg/mL.
Concentration 1 mg/mL
Buffer PBS, pH 7.4, containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing cycles.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.