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  • 产品名称：HumanIgMIsotypeControl

  • 产品型号：
  • 产品厂商：BioLeaf
  • 产品文档：
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简单介绍：
HumanIgMIsotypeControl
详情介绍：
Isotype IgM
Fragment Fab μ fragment
Cross-Reactivity (Details) Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum. No reaction was observed against anti-Human IgG F(c). Some light chain cross reactivity will occur with anti-Human IgG.
Purification HUMAN IgM (myeloma) Fab μ fragment was prepared from serum by a multi-step process which includes delipidation, selective precipitation, tandem molecular sieve chromatography and trypsin digestion followed by extensive dialysis against the buffer stated above.
Sterility Sterile filtered
Research Area Secondary Antibodies
Application Notes

Application Note: Monovalent Fab fragments of affinity-purified, secondary antibodies are offered to cover (block) the surface of immunoglobulins for double labeling primary antibodies from the same host species, or to block endogenous immunoglobulins in tissue sections or on cell surfaces. They can be used for these purposes because each Fab fragment has only a single antigen . HUMAN IgM (myeloma) Fab μ fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Specific conditions should be optimized by the user.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiry Date 12 months