产品详情
  • 产品名称:HumanIgGIsotypeControl

  • 产品型号:
  • 产品厂商:BioLeaf
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简单介绍:
HumanIgGIsotypeControl
详情介绍:
Isotype IgG
Fragment F(ab')2 fragement
Characteristics Purified Human IgG Fab'2 for use as a control or blocking reagent
Source: Normal Human Serum/Plasma was obtained from healthy donors of US origin.
Alternative Names: Human Immunoglobulin G Fab'2
Purification Purified
Purity > 95 % pure
Sterility 0.2 μm filtered
Background Fab'2 fragments of antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving intact some of the hinge region. Fab'2 fragments have two antigen-binding Fab portions linked together by disulfide bonds, and therefore are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of antibodies to live cells with Fc receptors or to Protein A or Protein G.
Synonyms: Human Immunoglobulin G Fab'2.
Molecular Weight 110 kDa
Research Area Immunology, Secondary Antibodies
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Clear, colorless liquid
Country of Origin: Normal Human Serum/Plasma was obtained from healthy donors of US origin.

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride and 0.05 % (w/v) Sodium Azide. Protease/IgG free.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles.
Storage 4 °C
Storage Comment Store at 2-8 °C.