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  • 产品名称：Rabbitanti-Mouse(Murine)IgGAntibody(HRP)

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  • 产品厂商：BioLeaf
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简单介绍：
Rabbitanti-Mouse(Murine)IgGAntibody(HRP)
详情介绍：
Immunogen

Immunogen: Highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins

Immunogen Type: Native Protein
Isotype IgG
Characteristics This Anti-Mouse IgG subclass pan reactive Secondary Antibody is is designed for Western Blotting, ELISA and Immunohistochemistry. HRP conjugated secondary antibodies can also be used for a variety of other applications such as Assay Development.
Purification Anti-Mouse IgG subclass pan reactive Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.
Research Area Immunology, Secondary Antibodies
Application Notes

Immunohistochemistry Dilution: 1:1000 - 1:5000

Application Note: When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.

Western Blot Dilution: 1:2000 - 1:10000

ELISA Dilution: 1:20,000 - 1:40,000
Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Gentamicin sulfate
Precaution of Use This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months