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Immunogen
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A pool of purified Bence Jones lambda proteins isolated from mouse urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Isotype
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IgG
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Specificity
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The reactivity of the antiserum is directed to the surface and hidden determinants of Ig lambda light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the lambda type, Bence Jones proteins as well as free light chains of the lambda type. This antiserum does not react with any other protein of mouse serum or plasmaPrecipitating polyclonal goat antiserum to free and bound mouse Ig lambda light chain
To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immuno-diffusion.
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Characteristics
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Precipitating polyclonal goat antiserum to free and bound mouse Ig lambda light chain
To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immuno-diffusion.
Physicochemical characteristic Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled goat serum. Antibody titre: Precipitin titre not less than 1:32 when tested against normal mouse serum
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Purification
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Delipidated, heat inactivated lyophilized whole serum.
Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with mouse serum proteins.
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