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  • 产品名称：Goatanti-HumanIgAAntibody(AlkalinePhosphatase(AP))

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  • 产品厂商：BioLeaf
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简单介绍：
Goatanti-HumanIgAAntibody(AlkalinePhosphatase(AP))
详情介绍：
Immunogen

Immunogen: Human IgA alpha heavy chain
Isotype IgG
Fragment Fab fragment
Characteristics Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Human IgA and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Research Area Immunology, Secondary Antibodies
Application Notes

Immunohistochemistry Dilution: 1:200-1:1,000

Application Note: This product has been assayed against 1.0 μg of Human IgA in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500-1:2,500

ELISA Dilution: 1:2,000-1:10,000
Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 500 μL
Concentration 0.6 mg/mL
Buffer

Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Stabilizer: 15 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted powder. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Expiry Date 12 months