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  • 产品名称:Goatanti-FerretIgAAntibody(HRP)

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  • 产品厂商:BioLeaf
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简单介绍:
Goatanti-FerretIgAAntibody(HRP)
详情介绍:
Immunogen

Immunogen: Anti-Ferret IgA was produced by repeated immunization with ferret IgA alpha heavy chain in goat.

Isotype IgG
Fragment Fab fragment
Characteristics Anti-Ferret IgA (alpha chain) Antibody detects specifically ferret IgA (alpha chain). This secondary Anti-Ferret IgA Antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Ferret IgA (alpha chain) Antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
Purification Anti-Ferret IgA (alpha chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Ferret IgA and Ferret Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Ferret heavy or light chain isotypes.
Research Area Immunology, Secondary Antibodies
Application Notes

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Ferret IgA (alpha chain) antibody has been assayed against 1.0 μg of Ferret IgA in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for Anti-Ferret IgA (alpha chain) Antibody.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months