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Immunogen
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Highly purified secretory IgA isolated from pooled swine milk. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Isotype
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IgG
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Specificity
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The reactivity of the antiserum is restricted to the Fc part of the IgA molecule and the surface and hidden determinants of the secretory component. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal swine milk two precipitin lines may be obtained, one representing IgA and the other the free secretory component. Against normal swine serum only IgA is precipitated. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments
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Cross-Reactivity (Details)
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The antiserum does not cross-react with any other component of the swine immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.
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Characteristics
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Precipitating polyclonal rabbit antiserum to swine secretory IgA
Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against pooled normal swine serum in agar-block immuno-diffusion titr
Delipidated, heat inactivated, lyophilized, stable whole antiserum
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Purification
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Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
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