Immunogen
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Purified normal IgG and fractions containing IgA and IgM and isolated from cat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Isotype
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IgG
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Specificity
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The reactivity of the antiserum is directed to the major isotypes of the cat immunoglobulin system as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulinsPrecipitating polyclonal goat antiserum to cat immunoglobulins, heavy and light chains
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Characteristics
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Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. Antibody titre: Precipitin titre not less than 1:64 when tested against normal cat serum in agar block titration.
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Purification
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Delipidated, heat inactivated, lyophilized stable whole serum Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity with other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
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