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  • 产品名称：Mouseanti-HumanIgEAntibody

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIgEAntibody
详情介绍：
Immunogen Highly purified monoclonal IgE isolated from human serum
Clone NI 307
Isotype IgG1 kappa
Specificity The reactivity of the antiserum is restricted to the IgE molecule as tested in ELISA, immunoblotting, immunoprecipitation. Precipitation takes place under special test conditions in the presence of polyethylene glycol. Purified monoclonal mouse antibody to human IgE, isotype specific
Characteristics Purified monoclonal mouse antibody to human IgE, isotype specific
Purification Purified
Research Area Immunology, Secondary Antibodies
Application Notes To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining of cytoplasmic IgE immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100, in ELISA from 1:50 upwards, in Western blotting from 1:100 upwards. These data should be interpreted as general recommendations only.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.
Concentration 0.4 mg/mL
Buffer Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
Handling Advice Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
Storage 4 °C/-20 °C
Storage Comment The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.