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  • 产品名称：Mouseanti-HumanIg(Chainlambda),(Free&Bound)Antibody

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIg(Chainlambda),(Free&Bound)Antibody
详情介绍：
Immunogen Highly purified Bence Jones lambda proteins isolated from pooled human urine.
Clone NI 412 and NI 268
Isotype IgG1 kappa
Characteristics The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the lambda type, as well as free lambda light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immuno-peroxidase staining. Purified monoclonal mouse antibody to human Ig lambda chain, free and bound
Purification Purified
Application Notes To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, indirect immunoperoxidasestaining and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:400 upwards. These data should be interpreted as general recommendations only.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
Concentration 0.4 mg/mL
Buffer Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
Preservative Without preservative
Handling Advice Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.
Storage 4 °C/-20 °C
Storage Comment The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 24 °C.