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  • 产品名称：Mouseanti-HumanIgG(FcRegion)Antibody

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIgG(FcRegion)Antibody
详情介绍：
Immunogen Highly purified IgG isolated from pooled human serum
Clone NI 335 and NI 343
Isotype IgG1 kappa
Specificity The reactivity of the antiserum is restricted to isotype specific determinants on the Fc part of the IgG molecule. The antiserum reacts with the 4 subclasses of IgG, as tested in indirect binding enzyme immunoassay, immunoblotting and indirect immuno-peroxidase staining of cytoplasmic Ig. Monoclonal mouse antiserum to human IgG, isotype specific
Purification Delipidated, heat inactivated, lyophilized, stable whole ascites
Research Area Immunology, Secondary Antibodies
Application Notes To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.
Concentration 1.0 mg/mL
Buffer No foreign proteins added.
Handling Advice Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
Storage 4 °C/-20 °C
Storage Comment The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.