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  • 产品名称：Goatanti-HumanIg(Chainkappa),(LightChain)Antibody(AlkalinePhosphatase(AP))

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  • 产品厂商：BioLeaf
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简单介绍：
Goatanti-HumanIg(Chainkappa),(LightChain)Antibody(AlkalinePhosphatase(AP))
详情介绍：
Immunogen Purified human kappa (kappa) light chain
Specificity Based on IEP, this antibody reacts with: kappa (κ) light chains on all human immunoglobulins
Cross-Reactivity (Details) Based on IEP, no reactivity is observed to: non-immunoglobulin human serum immunoglobulins and heavy chains on all human immunoglobulins and lambda (λ) light chains on all human immunoglobulins
Characteristics Concentration: 1.50 mg/mL (E 1 % at 280 nm = 13.0)
Conjugate: Alkaline Phosphatase
Purification Affinity purified using solid phase human Kappa (κ) Chain
Purity > 95 % based on SDS-PAGE
Alternative Name Immunoglobulin Ig
Application Notes This Conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications
Comment

ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm
Restrictions For Research Use only
Format Liquid
Concentration 1.50 mg/mL
Buffer 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Prepare working dilution prior to use and then discard.
Storage 4 °C
Storage Comment Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.