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  • 产品名称：Goatanti-Rat(Rattus)IgGAntibody(FITC)

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  • 产品厂商：BioLeaf
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简单介绍：
Goatanti-Rat(Rattus)IgGAntibody(FITC)
详情介绍：
Immunogen

Immunogen: Rat IgG F(ab')2 fragment
Isotype IgG
Fragment Fab fragment
Cross-Reactivity (Details) Pre-Adsorbed
Characteristics This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgG F(ab')2 and Rat Serum. No reaction was observed against Rat IgG F(c), anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse and Human Serum Proteins.
ProductDetails: Labeling Ratio 2.6
Research Area Immunology, Secondary Antibodies
Application Notes

Flow Cytometry Dilution: 1:500 - 1:2,500

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Western Blot Dilution: 1:50 - 1:200

FLISA Dilution: 1:50 - 1:200

IF Microscopy Dilution: 1:50 - 1:200
Comment

This product has been overfilled to ensure total recovery of stated quantity.
Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)
Concentration 1.5 mg/mL
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: 15 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months