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  • 产品名称:Goatanti-Rat(Rattus)IgG(Heavy&LightChain)Antibody(HRP)

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  • 产品厂商:BioLeaf
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简单介绍:
Goatanti-Rat(Rattus)IgG(Heavy&LightChain)Antibody(HRP)
详情介绍:
Immunogen Anti-Rat IgG (H&L) was produced by repeated immunization with rat IgG whole molecule in goat.
Immunogen Type: Native Protein
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum.
Characteristics This secondary peroxidase conjugated antibody anti-Rat is ideal for investigators who routinely perform titration assays, western blot, immunoprecipitation and more generally immunoassays.
Purification Anti-Rat IgG (H&L) peroxidase conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
Background Synonyms: Rat IgG (H&L) Antibody, Fab Gt-a-Rat Fab Rat IgG Peroxidase Antibody in goat, Goat Fab peroxidase conjugated Rat Secondary Antibody
Research Area Immunology, Secondary Antibodies
Application Notes Anti-Rat IgG HRP antibody is uitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Gentamicin sulfate
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
Expiry Date 12 months