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  • 产品名称：Piganti-Horse(Equine)IgG(LightChain),(Free&Bound)Antibody

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  • 产品厂商：BioLeaf
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简单介绍：
Piganti-Horse(Equine)IgG(LightChain),(Free&Bound)Antibody
详情介绍：
Immunogen Purified equine light chains isolated from polyclonal horse IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Specificity As tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal horse serum and purified IgG fractions and light chain preparations, the antiserum reacts with determinants exposed on the surface (SD) and the Fab portion of all intact immunoglobulins, with Fab or F(ab')2 subunits, and with free light chains of both types, also with determinants exposed only on free light chains but hidden in the intact immunoglobulin molecule (HD or hidden determinants) or Bence Jones proteins. This antiserum is not kappa or lambda chain specific.
Characteristics Precipitating polyclonal swine antiserum to horse light chains, free and bound
Total protein and IgG concentration in the antiserum are comparable to those of pooled swine serum. Antibody titre: Precipitin titre not less than 1:64 when tested against normal horse serum in agar block titration.
Purification Delipidated, heat inactivated lyophilized stable whole serum
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies reacting with the Fc fragments of immunoglobulins or with other plasma proteins.
Research Area Immunology, Secondary Antibodies
Application Notes As precipitating antiserum in a variety of immunodiffusion techniques including immuno-electrophoresis and double and single radial immunodiffusion Mancini, Ouchterlony. It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.
Restrictions For Research Use only
Format Lyophilized
Reconstitution It is reconstituted by adding 1 mL sterile distilled water.
Buffer No foreign proteins added.
Preservative Without preservative
Handling Advice Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably use d the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Storage 4 °C/-20 °C
Storage Comment The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.