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  • 产品名称：Rabbitanti-Mouse(Murine)IgG(Heavy&LightChain)Antibody(HRP)

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  • 产品厂商：BioLeaf
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简单介绍：
Rabbitanti-Mouse(Murine)IgG(Heavy&LightChain)Antibody(HRP)
详情介绍：
Immunogen Mouse IgG whole molecule
Fragment F(ab')2 fragment
Specificity F(ab')2 Anti-Mouse IgG (H&L)Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Characteristics F(ab')2 Anti-Mouse IgG antibody is ideal for investigators involved in serum component protein research.
The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Purification Immunoaffinity Chromatography.
Research Area Immunology, Secondary Antibodies
Application Notes F(ab') 2 Anti-Mouse IgG (H&L) has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Specific conditions for reactivity should be optimized by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
Concentration 0.5 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Gentamicin sulfate
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage -20 °C
Storage Comment Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
Expiry Date 12 months