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  • 产品名称：Sheepanti-RabbitIgG(Heavy&LightChain),(Chaingamma)Antibody(HRP)

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  • 产品厂商：BioLeaf
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简单介绍：
Sheepanti-RabbitIgG(Heavy&LightChain),(Chaingamma)Antibody(HRP)
详情介绍：
Immunogen Purified Rabbit IgG, whole molecule
Specificity Based on IEP, this antibody reacts with: heavy (gamma) chains on rabbit IgG, light chains on all rabbit immunoglobulins
No Cross-Reactivity Rabbit
Purification Affinity purified using solid phase Rabbit IgG (H and L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for all immunoassay applications.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:5,000 for Immunohistochemistry. 1:200-1:5000 for ELISA/Western blot
Comment

Country of Origin: Sheep serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
HRP: Enzyme derived from the plant horseradish, A small molecule that does not cause steric hindrance in antibody-antigen reactions, Highly sensitive, can detect low levels of antibody and can be used in a sensitive ECL assay, Hydrogen peroxide oxidizes chromogenic substrates such as TMB, ABTS, and OPD to produce a soluble product with a distinct color which can be measured by spectrophotometer, The reaction product has a high color stability after the reaction is stopped. Detection OD of 450 nm
Restrictions For Research Use only
Format Lyophilized
Reconstitution Rehydrate with 1.1 mL of deionized water. Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution. If necessary, Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Concentration 1.0 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative Kathon CG
Handling Advice Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage 4 °C