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  • 产品名称：Sheepanti-Mouse(Murine)IgG(FabRegion),(Chainkappa),(Chainlambda),(Free&Bound)An

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  • 产品厂商：BioLeaf
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简单介绍：
Sheepanti-Mouse(Murine)IgG(FabRegion),(Chainkappa),(Chainlambda),(Free&Bound)Antibody
详情介绍：
Immunogen Purified Fab of normal mouse IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Specificity The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab')2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion
Characteristics Precipitating polyclonal sheep antiserum to mouse Fab of IgG
Total protein and IgG concentration in the antiserum are comparable to those of pooled sheep serum. Antibody titre: Precipitin titre not less than 1:64 when tested against normal mouse serum in agar block titration.
Purification Delipidated, heat inactivated lyophilized stable whole serum
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies reacting with other subunits of the immunoglobulin or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of foreign protein or immune complexes in the antiserum.
Research Area Immunology, Secondary Antibodies
Application Notes In precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of Fab, free or bound.
Restrictions For Research Use only
Format Lyophilized
Reconstitution It is reconstituted by adding 1 mL sterile distilled water.
Buffer No foreign proteins added.
Preservative Without preservative
Handling Advice Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Storage 4 °C/-20 °C
Storage Comment The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.