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  • 产品名称：Mouseanti-HumanIgEAntibody(HRP)

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIgEAntibody(HRP)
详情介绍：
Immunogen Highly purified monoclonal IgE isolated from human serum.
Clone NI 307
Isotype IgG1 kappa
Characteristics The reactivity of the this monoclonal antibody is restricted to polyclonal and monoclonal IgE, as tested in direct binding enzyme immunoassay, immuno-blotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic immunoglobulins. Horseradish peroxidase conjugated purified monoclonal mouse antibody to human IgE, isotype specific
Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).
Purification Purified
Research Area Immunology, Secondary Antibodies
Application Notes To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:250, in ELISA from 1:250 upwards, in Western blotting from 1:500 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
Concentration 0.4 mg/mL
Buffer Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH 7.2).
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage 4 °C/-20 °C
Storage Comment The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.