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  • 产品名称：HumanAllergenProteinArrayG1

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  • 产品厂商：RayBiotech
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简单介绍：
HumanAllergenProteinArrayG1
详情介绍：
Purpose Human Allergen Protein Array (glass slide) contains 48 common allergen proteins for simultaneous detection of human allergens from foods, animals, trees, grass, pollen, mites, fungi (molds), and insects.
Sample Type Serum
Analytical Method Semi-Quantitative
Detection Method Fluorometric
Specificity See Product Page
Characteristics
  • Simultaneous detection of 48 allergen proteins
  • High sensitivity
  • Large dynamic range
  • Low sample volume required (10 - 100 μL per array)
  • Suitable for high-throughput assays
  • High efficiency and accuracy
  • Affordable, quick and simple to use
  • Customizable: Tell us the allergens you're interested in
  • Test 4 or 16 samples on each slide
Components Human Allergen Protein Array Glass Slide
G series antibody array accessory (including slide incubation chamber assembly, Gasket, Protective cover, Snap-on sides and adhesive film)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotin-conjugated anti-human IgE
Biotin-conjugated anti-human IgG
Cy3-Conjugated Streptavidin
Material not included Orbital shaker or oscillating rocker
Pipettors, pipette tips and other common lab consumables
Small plastic boxes or containers
Distilled water
Aluminum foil
Laser scanner for fluorescence detection
Comment

The Allergen Protein Arrays are semi-quantitative multiplex protein immunoassays developed for the detection of common allergens. Recombinant allergen proteins or extracted native allergen proteins are spotted onto a solid glass slide surface, and then probed for the presence of serum specific antibodies to these allergen targets. These detection kits can then be applied in screening specific allergens based on IgE and/or IgG levels present in patient serum samples.
Sample Volume 25 μL
Plate Glass Slide
Protocol Sample incubation (1-2 hours)Biotin-conjugated antibody incubation (1-2 hours)Cy3-Streptavidin incubation (1 hour)Scan and data analysis
Restrictions For Research Use only
Handling Advice Do not touch the surface of the slides, as the microarray slides are very sensitive. Hold the slides by the edges only. Handle all buffers and slides with powder free gloves. Handle glass slide/s in clean environment. The G-Series slides do not have bar codes. To help distinguish one slide from another, transcribe the slide serial number from the slide bag to the back of the slide with a fine point permanent marker. Please write the number on the very bottom edge of the slide, taking care to avoid writing on the array well areas.
Storage -20 °C/-80 °C
Storage Comment Upon receipt, all components of the RayBio® Human Allergen Protein Array kit should be stored at -20 °C to -80 °C until just before the experiment. At -20 °C to -80 °C the kit will retain complete activity for up to 6 months. Please use within six months of purchase. Once thawed, the array glass chip and Blocking Buffer should be kept at -20 °C and all other component should be stored at 4 °C Use within three months after reagents have been thawed.