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  • 产品名称：Rabbitanti-Mouse(Murine)IgM(FcRegion)Antibody

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  • 产品厂商：BioLeaf
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简单介绍：
Rabbitanti-Mouse(Murine)IgM(FcRegion)Antibody
详情介绍：
Immunogen Highly purified homogenous IgM isolated from mouse serum. Immunization with intact (19S) and split IgM (7S). Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Specificity The defined antibody specificity is restricted to the Fc part of the IgM molecule, as tested against mouse sera at the level of sensitivity of direct immunofluorescence staining methods applied to lymphoid cells with the conjugated form of this product. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations, against mouse serum a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with the purified IgM.
Characteristics Purified IgG fraction of polyclonal rabbit antiserum to mouse IgM, Fc specific
Antibody titre: Precipitin titre not less than 1:64 when tested against normal mouse serum in agar-block immunodiffusion titration. Performance testing Titre, specificity and reactivity of the
Purification Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the elimination of antibodies to the common Ig/Fab portion. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
Research Area Secondary Antibodies
Application Notes As unlabelled primary or secondary antibody reagent for indirect detection of IgM in mouse cells, tissues and body fluids in immunofluorescence and immunoenzyme assay methods, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling techniques, the optimum concentration of this product should be established before. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays are between 1:1,000 and 1:5,000.
Restrictions For Research Use only
Format Lyophilized
Reconstitution It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
Concentration 10 mg/mL
Buffer Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
Preservative Without preservative
Handling Advice Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Storage 4 °C/-20 °C
Storage Comment The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged s torage at or below -24 °C.