Lane 1: Monkey IgG. Load: 100 ng per lane. Primary antibody: Monkey IgG gamma antibody at 1:1000 for overnight at 4 deg C. Secondary antibody: Dylight (TM) 800 Donkey anti-Goat at 1:20, 000 for 30 min at RT. Block for 30 min at RT. Size: 55 kDa; Other band(s): splice variants and isoforms.