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  • 产品名称：Mouseanti-HumanIg(Chainalpha)Antibody(Biotin)

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIg(Chainalpha)Antibody(Biotin)
详情介绍：
Clone IP26
Isotype IgG1
Specificity The mouse monoclonal antibody IP26 recognizes a monomorphic determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood.
Characteristics The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Alternative Name Immunoglobulin Ig
Background The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 1:300. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Kuttruff, Koch, Kelp, Pawelec, Rammensee, Steinle: "NKp80 defines and stimulates a reactive subset of CD8 T cells." in: Blood, Vol. 113, Issue 2, pp. 358-69, 2009 (PubMed).

Ortonne, Huet, Gaudez, Marie-Cardine, Schiavon, Bagot, Musette, Bensussan: "Significance of circulating T-cell clones in Sezary syndrome." in: Blood, Vol. 107, Issue 10, pp. 4030-8, 2006 (PubMed).

Huet, Bagot, Loyaux, Capdevielle, Conraux, Ferrara, Bensussan, Marie-Cardine: "SC5 mAb represents a unique tool for the detection of extracellular vimentin as a specific marker of Sezary cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 1, pp. 652-9, 2005 (PubMed).

Background publications Croxford, Akilli-Ozturk, Rieux-Laucat, Förster, Waisman, Buch: "MHC-restricted T cell receptor signaling is required for alpha beta TCR replacement of the pre T cell receptor." in: European journal of immunology, Vol. 38, Issue 2, pp. 391-9, 2008 (PubMed).

Levelt, Wang, Ehrfeld, Terhorst, Eichmann: "Regulation of T cell receptor (TCR)-beta locus allelic exclusion and initiation of TCR-alpha locus rearrangement in immature thymocytes by signaling through the CD3 complex." in: European journal of immunology, Vol. 25, Issue 5, pp. 1257-61, 1995 (PubMed).

von Boehmer: "T cell development and selection in the thymus." in: Bone marrow transplantation, Vol. 9 Suppl 1, pp. 46-8, 1992 (PubMed).