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  • 产品名称：Mouseanti-HumanIg(Chainalpha)Antibody(FITC)

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIg(Chainalpha)Antibody(FITC)
详情介绍：
Clone IP26
Isotype IgG1
Specificity The mouse monoclonal antibody IP26 recognizes a monomorphic determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood.
Characteristics The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Alternative Name Immunoglobulin Ig
Background The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Product cited in: Kuttruff, Koch, Kelp, Pawelec, Rammensee, Steinle: "NKp80 defines and stimulates a reactive subset of CD8 T cells." in: Blood, Vol. 113, Issue 2, pp. 358-69, 2009 (PubMed).

Ortonne, Huet, Gaudez, Marie-Cardine, Schiavon, Bagot, Musette, Bensussan: "Significance of circulating T-cell clones in Sezary syndrome." in: Blood, Vol. 107, Issue 10, pp. 4030-8, 2006 (PubMed).

Background publications Croxford, Akilli-Ozturk, Rieux-Laucat, Förster, Waisman, Buch: "MHC-restricted T cell receptor signaling is required for alpha beta TCR replacement of the pre T cell receptor." in: European journal of immunology, Vol. 38, Issue 2, pp. 391-9, 2008 (PubMed).

Levelt, Wang, Ehrfeld, Terhorst, Eichmann: "Regulation of T cell receptor (TCR)-beta locus allelic exclusion and initiation of TCR-alpha locus rearrangement in immature thymocytes by signaling through the CD3 complex." in: European journal of immunology, Vol. 25, Issue 5, pp. 1257-61, 1995 (PubMed).

von Boehmer: "T cell development and selection in the thymus." in: Bone marrow transplantation, Vol. 9 Suppl 1, pp. 46-8, 1992 (PubMed).