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  • 产品名称：Mouseanti-HumanIgG(FcRegion)Antibody

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIgG(FcRegion)Antibody
详情介绍：
Isotype IgG1
Specificity Chrompure Human IgG, Fc Fragment
Characteristics Monoclonal Anti-Human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor
Purification Antibodies are purified by protein A affinity chromatography
Gene ID 2209
Research Area Immunology, Secondary Antibodies
Application Notes Western Blot: 1:200- 1:1,000
ELISA: Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Product cited in: Klonisch, Panayotou, Edwards, Jackson, Berger, Delves, Lund, Roitt: "Enhancement in antigen binding by a combination of synergy and antibody capture." in: Immunology, Vol. 89, Issue 2, pp. 165-71, 1996 (PubMed).