Western Blot of Rabbit anti-Swine antibody. Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: Swine antibody at 1:1,000 for overnight at 4°C. Secondary antibody: DyLight™ swine secondary antibody at 1:20,000 for 30 min at RT Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa for Swine IgG. Other band(s): none.