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  • 产品名称:Piganti-HumanIg(Chainkappa)Antibody(FITC)

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  • 产品厂商:BioLeaf
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简单介绍:
Piganti-HumanIg(Chainkappa)Antibody(FITC)
详情介绍:
Immunogen A pool of purified Bence Jones kappa proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Specificity The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of human serum or plasmaFluorescein isothiocyanate-conjugated IgG fraction of polyclonal swine antiserum to free and bound human Ig kappa light chain
Characteristics Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal swine antiserum to free and bound human Ig kappa light chain
Fluorochrome/IgG protein ratio (F/P) approximately 2.8.
Purification Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
Application Notes This conjugate is intended for use in direct immunofluorescence for the detection of polyclonal immunoglobulins, purified monoclonal immunoglobulins of the kappa type as well as free light chains or Bence Jones proteins of the kappa type in serum or other body fluids. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.
Comment

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Format Lyophilized
Reconstitution It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
Concentration 10 mg/mL
Buffer Fluorochrome coupled purified swine IgG lyophilized from a solution in phosphate buffered saline (pH 7.2).
Preservative Without preservative
Handling Advice Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably use d the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Storage 4 °C/-20 °C
Storage Comment The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.