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  • 产品名称：Goatanti-HumanIgA,IgG,IgM(FabRegion)Antibody(HRP)

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  • 产品厂商：BioLeaf
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简单介绍：
Goatanti-HumanIgA,IgG,IgM(FabRegion)Antibody(HRP)
详情介绍：
Immunogen Purified polyclonal human IgG, and IgA and IgM containing factions isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Specificity The reactivity of the antiserum is directed to the major isotypes of the human immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface deter-minants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immuno-diffusion using various antiserum concentrations against normal human plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained
Characteristics Horseradish peroxidase-conjugated IgG fraction of polyclonal goat antiserum to human IgG, IgA and IgM, heavy and light chains
Peroxidase/IgG protein molar ratio (E/P) is approximately 1.4. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).
Purification Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation and purification of the IgG (7S) fraction containing the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis.
Alternative Name IgG + IgA + IgM
Application Notes In enzyme-immunocytochemical and immunohistochemical staining for the detection of cytoplasmic Ig at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,500 and 1:15,000.
Restrictions For Research Use only
Format Lyophilized
Reconstitution It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
Concentration 11.2 mg/mL
Buffer Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
Preservative Without preservative
Handling Advice Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, a nd preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Storage 4 °C/-20 °C
Storage Comment The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.