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  • 产品名称:Piganti-RabbitIgG(Heavy&LightChain)Antibody

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  • 产品厂商:BioLeaf
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简单介绍:
Piganti-RabbitIgG(Heavy&LightChain)Antibody
详情介绍:
Immunogen Purified normal IgG isolated from pooled rabbit serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Specificity The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.
Characteristics Antibody titre: Precipitin titre not less than 1:128 when tested against normal rabbit serum in agar block titration.
Purification No adsorption required. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
Research Area Immunology, Secondary Antibodies
Application Notes In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal human serum, as bridging reagent in a peroxidase antiperoxidase technique using R/PAP, to prepare an immuno-adsorbent for the production of Ig-free rabbit serum, plasma or non-Ig preparations.
Restrictions For Research Use only
Format Lyophilized
Reconstitution It is reconstituted by adding 1 mL sterile distilled water.
Concentration 10 mg/mL
Buffer Purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
Preservative Without preservative
Handling Advice Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same da y. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Storage 4 °C/-20 °C
Storage Comment The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.