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  • 产品名称：Mouseanti-HumanIgEAntibody(TRITC)

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIgEAntibody(TRITC)
详情介绍：
Immunogen Highly purified IgE isolated from human serum.
Clone NI 307
Isotype IgG1 kappa
Specificity The reactivity of the antiserum is restricted to a isotype specific determinant of the IgE molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunofluorescence staining of cytoplasmic Ig. Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgE, isotype specific
Characteristics Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgE, isotype specific
Fluorochrome/IgG protein molar ratio (F/P) approximately 2.4.
Purification Purified
Research Area Immunology, Secondary Antibodies
Application Notes To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgE and cell membrane IgE.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100. These data should be interpreted as general recommendations only.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
Concentration 0.4 mg/mL
Buffer Purified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH 7.2).
Preservative Without preservative
Handling Advice Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
Storage 4 °C/-20 °C
Storage Comment The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.