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  • 产品名称:Mouseanti-HumanIgMAntibody(FITC)

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  • 产品厂商:BioLeaf
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简单介绍:
Mouseanti-HumanIgMAntibody(FITC)
详情介绍:
Immunogen Highly purified IgM isolated from pooled human serum
Clone NI 179
Isotype IgG1 kappa
Specificity The reactivity of the antiserum is restricted to an isotype specific determinant of the IgM molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunofluorescence staining of cytoplasmic Ig. Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgM, isotype specific
Characteristics Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgM, isotype specific
Fluorochrome/IgG protein molar ratio (F/P) approximately 1.4.
Purification Purified
Research Area Secondary Antibodies
Application Notes To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immuno-fluorescence staining of cytoplasmic IgM and cell membrane IgM.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50. These data should be interpreted as general recommendations only.
Comment

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilize d product by adding 0. 5 mL sterile distilled water.
Concentration 0.4 mg/mL
Buffer Purified monoclonal mouse IgG1 kappa conjugated with fluorescein isothiocyanate, lyophilized from a solution in phosphate buffered saline (pH 7.2).
Preservative Without preservative
Handling Advice Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
Storage 4 °C/-20 °C
Storage Comment The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.