产品详情
所在位置: 首页> 产品目录> 二抗>
查看大图

  • 产品名称：Mouseanti-HumanIgMAntibody(Biotin)

  • 产品型号：
  • 产品厂商：BioLeaf
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
Mouseanti-HumanIgMAntibody(Biotin)
详情介绍：
Immunogen Highly purified polyclonal IgM isolated from human serum.
Clone NI 179
Isotype IgG1 kappa
Specificity The reactivity of the antiserum is restricted to an isotype specific determinant of the IgM molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig. Biotin-conjugated purified monoclonal mouse antibody to human IgM, isotype specific
Characteristics Biotin/IgG protein molar ratio (B/P) approximately 7.1.
Purification Purified
Research Area Secondary Antibodies
Application Notes To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgM, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:100 to 1:500, in ELISA from 1:1,000 upwards, in Western blotting from 1:2,000 upwards. These data should be interpreted as general recommendations only. Working dilutions may vary widely, strongly depending on the test conditions.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
Concentration 0.4 mg/mL
Buffer Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH 7.2).
Preservative Without preservative
Handling Advice Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
Storage 4 °C/-20 °C
Storage Comment The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.