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  • 产品名称：Mouseanti-HumanIgG3Antibody(HRP)

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIgG3Antibody(HRP)
详情介绍：
Immunogen Highly purified monoclonal IgG3 isolated from pooled human serum
Clone NI 330 (HP 6050)
Isotype IgG1 kappa
Specificity The reactivity of the antiserum is restricted to the subclass specific determinant on the F(ab')2 portion of the IgG3 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. Peroxidase conjugated purified monoclonal mouse antibody to human IgG3, subclass specific
Characteristics Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).
Purification Purified
Research Area Secondary Antibodies
Application Notes To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
Concentration 0.4 mg/mL
Buffer Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH 7.2).
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage 4 °C/-20 °C
Storage Comment The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.