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  • 产品名称：Sheepanti-HumanIgG(FcRegion)Antibody

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  • 产品厂商：BioLeaf
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简单介绍：
Sheepanti-HumanIgG(FcRegion)Antibody
详情介绍：
Immunogen Human IgG (Fd): Purified from pooled human serum
Specificity Has been shown to be specific by gel diffusion techniques.
Cross-Reactivity (Details) The cross-reactivity of this product against the sera of the species listed below has been assessed by double diffusion, using 10ul of antisera against 1ul of species sera. These results are intended as a guideline only. It is possible that cross-reactivity against species listed as negative below may be observed when using different test conditions or techniques.
Characteristics Sheep anti Human IgG (Fd), Sheep antibody to Human Immunoglobulin G (IgG), Fd specific
Purification Purified
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for use in a variety of gel techniques including radial immunodiffusion (RID), double diffusion and immunoelectrophoresis (IEP). The use of 3 % PEG 6000 with 1.3 % agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. RID: 10 µL antiserum/cm2 in gel vs 5ul human serum, 1:3 - 1:10 Double Diffusion: 10 µL antiserum vs 1ul human serum IEP: 100ul antiserum vs 1ul human serum
Restrictions For Research Use only
Format Liquid
Buffer Glycine buffered saline, pH 7.4, 0.099 % Sodium azide, 0.9 % EACA, 0.09 % Benzamidine, and 1 mM Ethylenediaminetetraacetic acid
Preservative Sodium azide, EACA, Benzamidine
Precaution of Use This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Storage 4 °C
Storage Comment Store at 2-8 °C. Slight precipitation can occur on storage which may be removed by centrifugation, and should not affect performance characteristics.