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Characteristics
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The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 1 normal human lung tissue, the cell line A549 (human lung carcinoma), and 7 different human lung tumor tissue lysates with 15 μg of total cellular protein in each lane. Proteins in a wide range of molecular mass are separated in a 4-20 % gradient SDS-PAGE gel and transferred onto nitrocellulose membranes. The lung tumor tissues included in the blot are 1 small cell carcinoma, 1 non-small cell carcinoma, 1 differentiated adenocarcinoma, 1 squamous cell carcinoma, 1 pleomorphic carcinoma and 2 non-diagnosed carcinomas (from different donors).
Sample diagnosis: Tumor
Samples loaded: Lanes: 1: protein markers, 2: A549, 3: normal lung tissue, 4: small cell carcinoma, 5: non-small cell carcinoma, 6: differentiated adenocarcinoma, 7: squamous cell carcinoma, 8: pleo-morphic carcinoma, 9: non-diagnosed carcinoma: 10: non-diagnosed carcinoma.
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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