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Characteristics
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The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries the cell line A549 (human lung carcinoma), and 3 different human lung tumor tissue lysates and matched normal lysates (from non-tumor lung tissue from near the tumor) with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane. The lung tumor tissues included in the blot are 1 small cell carcinoma, 1 non-small cell carcinoma, and 1 squamous cell carcinoma.
Sample diagnosis: Tumor
Samples loaded: Lanes: 1: protein markers, 2: A549, 3: small cell carcinoma, 4: matched normal lung tissue, 5: non-small cell carcinoma, 6: matched normal lung tissue, 7: squamous cell carcinoma, and 8: matched normal lung tissue.
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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