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Characteristics
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The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human tumor tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane. The human tumor tissues included in the blot are brain, colon, kidney, liver, lung, pancreas, skin, spleen, and stomach. Sample diagnosis: Tumor Samples loaded: Lanes: 1: protein markers, 2: brain, 3: colon, 4: kidney, 5: liver, 6: lung, 7: pancreas, 8: skin, 9: spleen, and 10: stomach Quantity loaded per lane: 15 μg Gel percentage: Gradient 4-20 %
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