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Characteristics
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The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane.The human normal tissues included in the blot are left cerebellum, right cerebellum, frontal lobe, occipital lobe, parietal lobe, whole eye, spinal cord, temporal lobe, and thalamus.
Sample diagnosis: Normal
Samples loaded: Lanes: 1: protein markers, 2: left cerebellum, 3: right cerebellum, 4: frontal lobe, 5: occipital lobe, 6: parietal lobe, 7: whole eye, 8: spinal cord, 9: temporal lobe, and 10: thalamus.
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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