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  • 产品名称：Thioredoxin2(TXN2)(AA60-166)Peptide

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  • 产品厂商：BioLeaf
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简单介绍：
Thioredoxin2(TXN2)(AA60-166)Peptide
详情介绍：
Sequence MTTFNIQDGP DFQDRVVNSE TPVVVDFHAQ WCGPCKILGP RLEKMVAKQH GKVVMAKVDI DDHTDLAIEY EVSAVPTVLA MKNGDVVDKF VGIKDEDQLE AFLKKLIG
Characteristics Purified recombinant Human Thioredoxin 2 protein
Expression System: E.coli
Bioactivity: Specific activity is 3-4 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Molecular weight on SDS-PAGE will appear higher.
Purity > 95 % pure
Background Thioredoxin(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The Thioredoxin 2 may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. Recombinant Thioredoxin 2 was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: Thioredoxin 2, MTRX protein, MT-TRX protein, Thioredoxin2 protein, TXN 2 protein, Thioredoxin mitochondrial protein, Thioredoxin-2, Thioredoxin 2 protein, thioredoxin 2 precursor protein, mitochondrial thioredoxin protein, Thioredoxin -2, TRX2 protein, TRX 2 protein, Thioredoxin -2 protein, Thioredoxin 2, TXN2 protein
Molecular Weight 11 kDa (108 AA)
Research Area Cancer, Metabolism, Signaling
Application Notes Thioredoxin 2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
Assay Procedure
  1. Prepare a 1 mL reaction cocktail in a suitable container: The final concentrations are 1 mM Tris, 61 mM sodium phosphate, 1 mM DTT, 0.1 % (w/v) insulin, 1 mM EDTA.
    2. Add 4 µg of recombinant thioredoxin-2 into reaction cocktail and mix immediately.
    3. Incubate at 25 °C for 20 minutes.
    4. Record the increase in A650 nm for 15 minutes.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Supplied as a liquid in PBS, pH 7.4.
Handling Advice Avoid repeated freeze/thaw cycles.
Storage RT/-20 °C
Storage Comment Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
Supplier Images
SDS-PAGE (SDS) image for Thioredoxin 2 (TXN2) (AA 60-166) peptide (ABIN934974) Figure annotation denotes ug of protein loaded and % gel used.