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  • 产品名称：Donkeyanti-GoatIgGAntibody(TRITC)

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  • 产品厂商：BioLeaf
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简单介绍：
Donkeyanti-GoatIgGAntibody(TRITC)
详情介绍：
Immunogen Purified IgG isolated from pooled goat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.
Cross-Reactivity Cow (Bovine), Monkey, Sheep (Ovine), Human
No Cross-Reactivity Hamster, Chicken, Pigeon, Turkey
Characteristics Physicochemical characteristics: IgG protein concentration 10 mg/mL. Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.8.

Physical form: TRITC-coupled purified hyperimmune donkey IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Fluorescent marker: Tetramethylrhodamine isothiocyanate isomer R. It has an orange-red fluorescence.
Excitation: 554 nm, emission: 573 nm. To avoid nonspecific background staining, specially synthesized and exceptionally pure crystalline isomer R has been used instead of the usual racemic mixture. Although its fluorescence efficiency is less than of FITC, TRITC conjugates have the advantage of significantly less photo bleaching. This facilitates their use in quantitative cell-counting procedures.

Conjugation procedure: A proprietary technique for the binding to TRITC is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life.
Purification Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
Adsorption: No adsorption required.
Background To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two immunoconjugates with different labels but originating from the same host animal species are used for direct two-color staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Research Area Immunology, Secondary Antibodies
Application Notes Working dilutions are usually between 1:20 and 1:120.
Restrictions For Research Use only
Format Lyophilized
Reconstitution It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles.
Buffer Phosphate buffered saline (PBS, pH 7.2)
Preservative Without preservative
Handling Advice Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Storage 4 °C/-20 °C
Storage Comment The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.