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  • 产品名称：Rabbitanti-Cat(Feline)IgGAntibody(HRP)

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  • 产品厂商：BioLeaf
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简单介绍：
Rabbitanti-Cat(Feline)IgGAntibody(HRP)
详情介绍：
Immunogen Purified normal IgG isolated from pooled cat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.
Cross-Reactivity Human, Ferret, Rat (Rattus), Dog (Canine)
No Cross-Reactivity Duck, Pigeon, Chicken, Turkey, Goat
Characteristics Physicochemical characteristics: IgG protein concentration 10 mg/mL. Enzyme/IgG protein molar ratio (E/P) is approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ = 3.2).

Physical form: Peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate method, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life.
Purification Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
Adsorption: No adsorption required.
Background In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non- isotopic assay methodology (e. g. ELISA) to identify and measure a specific IgG in cat serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Research Area Immunology, Secondary Antibodies
Application Notes Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:50, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:20,000.
Restrictions For Research Use only
Format Lyophilized
Reconstitution It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
Buffer Phosphate buffered saline (PBS, pH 7.2)
Preservative Without preservative
Handling Advice Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +0 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Storage 4 °C/-20 °C
Storage Comment The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.