产品详情
查看大图

  • 产品名称：ProSAP-InteractingProtein1(PROSAPIP1)(AA1-700)protein(Histag)

  • 产品型号：
  • 产品厂商：BioLeaf
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
ProSAP-InteractingProtein1(PROSAPIP1)(AA1-700)protein(Histag)
详情介绍：
Sequence MAPADLASEG PKLEDPPAPH LFGKCPSGLI MAKLETLPVR ADPGRDPLLA FAPRPSELGP PDPRLTMGSV GSGVTHAQEF PMKSVGTRTG GGGNQGSFPG PRSGGSGANR ERPGRYPSED KVLANSLYLN GELRGSDHTD VCGNVVGSSG GSSSSGGSDK APPQYREPNH PPKLLTTSGK LDQCSEPLVR PSAFKPVVPK NFHSMQNLCP PQTNGTPEGR QGPAGLKGGL DKSRTMTPAG GSGGGLSDSG RNSLTSLPTY SSSYSQHLAP LSASTSHINR IGTAGYSSGS SGGGSGYQDL GTSDSGRASS KSGSSSSMGR SGHLGSGEGG NGGLPFAACS PPSPSALIQE LEERLWEKEQ EVAALRRSLE QSEAAVAQVL EERQKAWERE LAELRQGCSG KLQQVARRAQ RAQQGLQLQV LRLQQDKKQL QEEAAQLIRQ REELEDKVAV CQKEQADFLP RMEETKWEVC QKAGEISLLK QQLKDSQADV SQKLSEIVGL RSQLREGRAS LREKEEQLLS LRDSFGSKQA SLELSEGELP PACLKPALTP VDLVEPQEAL ASCESDEAKM RRQAGVAAAA SLVSVDGEVE AGGEGGTRAL RREVGRLQAE LAAERRARER QGASFAEERR VWLEEKEKVI EYQKQLQLSY VEMYQRNQQL ERRLRERGAA GGSSTPTPQH GEEKKAWTPS RLERIESTEI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Lzts3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Purification Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin free.
Grade Crystallography grade
Molecular Weight 75.9 kDa Including tag.
UniProt A2AHG0
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Comment

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
image for ProSAP-Interacting Protein 1 (PROSAPIP1) (AA 1-700) protein (His tag) (ABIN3130747) „Crystallography Grade“ protein due to multi-step, protein-specific purification process