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  • 产品名称：Mouseanti-HumanIg(Chainkappa),(LightChain)Antibody(FITC)

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIg(Chainkappa),(LightChain)Antibody(FITC)
详情介绍：
Immunogen Human IgG-kappa myeloma protein
Clone TB28-2
Isotype IgG1
Specificity The antibody TB28-2 recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains.
Characteristics The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Background Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 4 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (0.4 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Product cited in: Böttcher, Ritgen, Buske, Gesk, Klapper, Hoster, Hiddemann, Unterhalt, Dreyling, Siebert, Kneba, Pott et al.: "Minimal residual disease detection in mantle cell lymphoma: methods and significance of four-color flow cytometry compared to consensus IGH-polymerase chain reaction at initial staging and for ..." in: Haematologica, Vol. 93, Issue 4, pp. 551-9, 2008 (PubMed).

Karandikar, Aquino, McKenna, Kroft: "Transient myeloproliferative disorder and acute myeloid leukemia in Down syndrome. An immunophenotypic analysis." in: American journal of clinical pathology, Vol. 116, Issue 2, pp. 204-10, 2001 (PubMed).

Nakamura, Kubagawa, Cooper: "Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8522-6, 1992 (PubMed).

Kiyotaki, Cooper, Bertoli, Kearney, Kubagawa: "Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 12, pp. 4150-8, 1987 (PubMed).