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  • 产品名称：Rabbitanti-Sheep(Ovine)IgG(WholeMolecule)Antibody(FITC)

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  • 产品厂商：BioLeaf
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简单介绍：
Rabbitanti-Sheep(Ovine)IgG(WholeMolecule)Antibody(FITC)
详情介绍：
Immunogen Sheep IgG whole molecule
Fragment F(ab')2 fragment
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG and Sheep Serum.
Characteristics This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Purification This product is a F(ab')2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above.
Research Area Immunology, Secondary Antibodies
Application Notes This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Comment

Excitation/Emission wavelength: 494 nm/514 nm
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
Concentration 10.0 mg/mL
Buffer 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Thimerosal (Merthiolate)
Precaution of Use Biohazard Informations: This product contains thimerosal which is hazardous.
Handling Advice Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing.
Do NOT add Sodium Azide!
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20 °C or below after dilution.
Storage -20 °C
Storage Comment Store vial at -20 °C or below prior to opening.
Expiry Date 12 months